INTERNATIONAL CONGRESS ON RECENT ADVANCES IN SCIENCES AND TECHNOLOGY - Kuala Lumpur - Malaysia (2019-02-20)

Agro-morphological and genetic diversity of bottle gourd Lagenaria siceraria (Molina) Standl. landraces in Jordan
Agro-morphological and Genetic diversity analysis was undertaken in 19 geographically distant landraces of bottle gourd (Lagenaria siceraria) from Jordan using 23 inter-simple sequence repeat (ISSR) primers and 11 agro morphological traits (seed length, seed width, seed area, distances between tips of cotyledons, petiole length, Plant length, number of tendrils, tendril length, number of leaves, leaf length, leaf width). Analysis of variance (ANOVA) showed significant differences in all agro-morphological traits between studied landraces. Phenotypic coefficients of variance were higher than genotypic coefficients of variance in all the characters indicating the influence of environment on the expression of these characters. High heritability (>60%) estimates for seed width, seed area, plant length, number of leaves and leaf width suggesting that these traits were primarily under genetic control. High H2 and high GAM (Genetic advance as a percentage of mean) were found for plant length, number of leaves, leaf width and seed area, this could be explained by additive gene action. Plant height showed positive correlation with number of tendrils (r=0.86), number of leaves (r=0.67) and leaf length (r=0.74). The dendrogram based on morphological data clustered landraces into six main groups . A total of 246 amplified bands were obtained using 23 ISSR primers , of which 135 were polymorphic with an average of 53% band polymorphism. Jaccard’s similarity coefficient indicated that genetic distances ranged from 0.78 to 0.92 based on molecular analysis and ranged from 0.42 to 0.91 based on the morphological data. The dendrogram based on molecular data clustered landraces into five main groups. No association were observed between agromorphological clustering and molecular clustering. The information on the molecular and agro-morphological traits can be used in future bottle gourd breeding programs to improve nutritional value and produce higher yielding varieties.
Professor wesam al khateeb