3RD INTERNATIONAL CONGRESS ON TECHNOLOGY - ENGINEERING & SCIENCE - Kuala Lumpur - Malaysia (2017-02-09)

New Coumarins From Aerial Parts Of Ammi Majus L And Their Cytotoxic Activity.

Three new coumarins were isolated from the aerial parts of the wild medicinal plant Ammi majus, which was collected from western Asia (Saudi Arabia), including 5-isobutylcoumarin-6-C-glucoside (1), 6,7,9-Trimethoxy-3-(8'-methoxy-2'-oxo-2H-chromen-3-yl)-2H-furo[3,2-g]chromen-2(3H)-one (2) which, was isolated for first time and 6-hydroxy-3-(2-hydroxypropyl)-7-methoxy-4 methyl coumarin (3). The structures were determined by spectroscopic methods, mainly 1D- and 2D-NMR. In vitro cytotoxicity was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl- tetrazolium bromide (MTT) assay. results of anticancer activity showed that compound 2 and 3 had high activity on different cell lines, Compound 1 showed high activity (95.7%) against HCT116 at concentration 100 ug/ml, but displayed week activity against MCF7 and A549 cell line.
Eman Elsharkawy, Yasser Selim