3RD INTERNATIONAL CONGRESS ON TECHNOLOGY - ENGINEERING & SCIENCE - Kuala Lumpur - Malaysia (2017-02-09)

Biosynthesis Of Zinc Oxide Nanoparticles By Locally Isolated Yeast Strain For Biomedical Applications
There has been a great attention to the development of ‘green’ synthesis of inorganic nanoparticles due to their potential applications in biomedical uses. The ‘green’ method for nanoparticle synthesis, which is rapidly replacing traditional chemical synthesis, is of great interest because of eco-friendliness, economic views, feasibility and the wide range of applications in several areas such as nano-medicine and catalysis medicine. The present study relates to a ‘green’ method for preparing zinc oxide nanoparticles (ZnO NPs) by yeast strain and evaluation of their antimicrobial and anticancer activities. The biosynthesized ZnO nanoparticles was characterized using Fourier transform infrared (FTIR), ultraviolet-visible (UV-vis), X-ray diffraction (XRD), transmission electron microscope (TEM), and field emission scanning electron microscope (FESEM) analyses. Based on the results, the polydispersed ZnO nanoparticles were easily prepared by this ‘green’ method. The XRD results indicated that hexagonal ZnO NPs with a mean size of (23 ± 2.4nm) was crystalline in structures. Moreover, antibacterial tests were evaluated on four types of gram positive and negative bacteria such as Streptococcus pneumonia, Staphylococcus aureus, Helicobacter and E. coli by disc diffusion methods. The ZnO NPs was able to inhibit 10.40 mm to 13.50 mm size inhibition. Additionally, in vitro anticancer activity on human breast cancer cell lines (MCF-7) was investigated on an apoptosis pathway by Real-time PCR and it was shown that ZnO NPs has a great effect on 7 genes of MCF-7 cell lines such as, Akt1, PARP1, ERK1/2, P38, P53, Bcl2 and TNF. In addition, the biosynthesized ZnO NPs was shown to have excellent antibacterial activity, as compared to the chemically synthesized ZnO NPs.
Amin BoroumandMoghaddam, Rosfarizan Mohamad, Raha Abdul Rahim, Arbakariya Bin Ariff, Farideh Namvar