3RD INTERNATIONAL CONGRESS ON TECHNOLOGY - ENGINEERING & SCIENCE - Kuala Lumpur - Malaysia (2017-02-09)

Role Of Lysine Chelated Zinc In Biofortification And Growth Enhancement Of Maize Irrigated With Tannery Wastewater
Tannery wastewaters are mainly characterized by high salinity, high organic loading, and specific pollutants such as sulfide and chromium. In many areas of Pakistan, industrial wastewater is being used for the purpose of irrigation of vegetable. At low concentration, tannery wastewater showed a beneficial effect as it contains macro and micronutrient minerals. Lysine is an α-amino acid that is used in the biosynthesis of proteins in plants. While lysine chelated zinc (LZ) is a compound comprises of zinc and lysine. Many studies have declared the zinc (Zn) beneficial effects on plants under abiotic stress, while lysine is reported as essential amino acid for plant and human body. Uniform plants were treated with tannery wastewater with four different concentration of wastewater (0, 25%, 50%, 100%) and three different concentration of LZ (0, 12.5 mM, 25 mM). Plants were harvested at maturity level and the data regarding root/shoot length, stem, leave fresh and dry weight was assessed. Infrared gas analyzer was used for assessment of stomatal conductance, photosynthetic rate and transpiration rate. Chlorophyll a, chlorophyll b, total chlorophyll and carotenoids were determined spectrophotometrically. Anti-oxidant enzymes, hydrogen peroxide, amino acid and soluble protein in root and leaf were evaluated spectrophotometrically. Chromium and zinc contents in different parts of plant such as root, stem and leaf was determined and data was subjected to appropriate statistical analysis. Antioxidant defense system of maize plant was improved due to the presence of mineral in wastewater. While at 50% or above treatment plants showed negative behavior. Spray of lysine chelated zinc improved the plant growth parameters and antioxidant defense system by supporting the enzymatic activity such as superoxide dismutase, peroxide dismutase and catalase. Due to high concentration of tannery wastewater at 50% or above photosynthetic pigment was also disturbed. A sudden decline was observed. With the application of lysine chelated zinc, toxicity of tannery wastewater decreased and zinc content were increased in different parts of plants.
Shafaqat Ali, Rehan Ahmad, Muhammad Rizwan