3RD INTERNATIONAL CONGRESS ON TECHNOLOGY - ENGINEERING & SCIENCE - Kuala Lumpur - Malaysia (2017-02-09)

Synergestic Effect Of Hypertermia And Chemotherapy In The Presence Of Gold Nanoparticles On The Growth Reduction Of Breast Adenocarcinema In Balb/c Mouse Model
According to high mortality rate due to cancer and considerable prevalence of breast cancer new methods have been developed to guide chemotherapy to the tumor. In this study, the effect of hypertermia and chemotherapy drug doxorubicin in the presence of gold nanoparticles on the growth reduction of breast adenocarcinema in balb/c mouse model was evaluated. To create hyperthermia, ultrasound waves of 3 MHz was used. To investigate the increasing effects of gold nanoparticles in the effectiveness of hyperthermia in adenocarcinema tumors in mice Balb / c, animals were divided into 5 groups (n=5): control, Sham, 3 MHz continuous ultrasound waves with 2 W/cm2 intensity, gold nanoparticle injection (50 mg/kg), gold nanoparticle injection with 3 MHz continuous ultrasound waves with 2 W/cm2 intensity. Then, the synergistic effect of hyperthermia combined with chemotherapy drug in the presence of gold nanoparticles were studied. In this step, animals were divided into 4 groups (n=5) doxorubicin injection (5 mg/kg), doxorubicin injection with 3 MHz continuous ultrasound waves with 2 W/cm2 intensity, the gold nanoparticles loaded with the chemotherapy drug doxorubicin (2.5 mg/kg), Nano gold particles loaded with the chemotherapy drug doxorubicin irradiated with 3 MHz ultrasonic waves continuous ultrasound waves with 2 W/cm2 intensity. The gold nanoparticles and drug for intravenous injection and sonication for 7 minutes on a daily. During tumor volume was measured every 3 days for a month. During treatment, tumor volume was measured every 3 days for a month. Survival time was recorded and histopathological results of groups using Bloom-Richardson grading were obtained. According the growth of tumors, tumor relative volume changes and survival parameter were extracted after treatment of animals. After injection of gold nanoparticles and exposure to ultrasound waves, the accumulation and increase of 50 percent gold nanoparticles in tumor tissue was harvested (P<0.05). Percentage of tumor growth inhibition in irradiated treated groups with gold nanoparticles 48.89 percent, respectively, were shown an increase compared to the sham group. Statistical analysis showed, there is significant decrease in growth delay parameters in treated tumors with gold nanoparticle injection and 3 MHz continuous ultrasound waves relative to other groups (P<0.05). Exposure to ultrasound waves after injection of doxorubicin with loaded nanoparticles are shown, the effect of drug on the adenocarcinoma tumor growth was doubled in comparison with the control group and increased 4/3 drug accumulation in the tumor tissue. Percentage of tumor growth inhibition in irradiated treated groups with gold nanoparticles and loaded gold nanoparticles with doxorubicin is 55.98 percent compared to the sham group. The animal survival rate is about 1.5 times compared to the control group. Concurrent application of ultrasound waves irradiation in the presence of gold nanoparticles and loaded gold nanoparticles with doxorubicin compared with only medication, with other groups indicate a significant difference in breast adenocarcinoma tumor growth. Figure 1. SEM micrograph showed gold nanoparticles.
Keywords: Sonication, Targeted Therapy, Gold Nanoparticles, Adenocarcinema Tumor
S. Javaherinezhad, M. Mokhtari-Dizaji, F. Ghanati, S. Ghaffari Khaligh