6TH International Congress on Technology - Engineering - Kuala Lumpur3 - Malaysia (2018-07-19)

Antimicrobial Activity Of Oil-in-water Piper Betle Emulsion Against Streptococcus Mutans And Streptococcus Aureus

Piper betle leaves is widely used for medicinal purposes, and the extract and essential oils of Piper betle leaves possess antibacterial activity. The purpose of this research was to investigate antibacterial activity of oil-in-water (O/W) Piper betle emulsions against dental cariogenic bacteria, Streptococcus mutans and Staphylococcus aureus. The Piper betle emulsion was formulated using high-energy ultrasonication method using 20kHz probe type ultrasonic processor. The emulsions was prepared by mixing 1g Piper betle oil, tween 80 with final volume 200 ml distilled water. The antibacterial activity of formulated emulsion was evaluated in vitro and the minimal inhibitory concentration (MIC) was determined by the macrodilution method. The bacterial killing kinetic was also evaluated for this formulated emulsion. The minimum inhibition concentration (MIC) of the Piper betle emulsion for Streptococcus mutans was 2.5 mg/ml and Staphylococcus aureus was 5 mg/ml. The time kill kinetics study showed that the formulated emulsion can act as microbiostatic agents. The time–kill curves clearly showed the ability of the Piper betle emulsion can reduce the bacterial population with time. The area under the curve (AUC) for Piper betle emulsion against Streptococcus mutans and Staphylococcus aureus revealed that the number of bacterial cells was significantly (p < 0.05) reduced when compared to the control sample. The results proposed that, this novel formulated emulsion have a potential to develop as an antibacterial drug for example for treatment of oral diseases.
Lee Weng Foo, Eraricar Salleh, Khairunadwa Jemon