6TH International Congress on Technology - Engineering - Kuala Lumpur3 - Malaysia (2018-07-19)

Cold-active Microbial Cellulase: Novel Approach To Understand Mechanism And Its Applications In Food And Beverages Industry

Cellulose is a most abundant carbohydrate produced by the plants. Cellulose degradation is a complex process and catalyzed by a group of enzymes called cellulases that are commercially involve in food processing. Nowadays cold-active enzymes attracted worldwide attention due to less energy requirement for their optimal activity and easy inactivation in comparison to their meso and thermophilic counterparts. In this study, cold-active cellulase from well-studied psychrophilic bacterium Pseudoalteromonas haloplanktis is evaluated for its industrial application compared to the normally used mesophilic and thermophilic cellulases by in silico approach. The cellulase structure from different origin were subjected to docking studies with the substrate cellulose by using MVD. The binding energy for thermo, meso and psychrophilic cellulase enzymes were found to be -93.29, -75.54 and -126.60 KCals/mole, respectively. The results specify that psychrophilic cellulase enzyme from Pseudoalteromonas haloplanktis has more efficiency to bind with cellulose (Figure 1) in comparison to its mesophilic and thermophilic counterparts; and may find better commercial application in food and beverage industry.
Mohammed Kuddus, Ghazala Yunus