5TH International Congress on Technology - Engineering & Science - Kuala Lumpur - Malaysia (2018-02-01)

Study Of The Effect Of Pinus Gerardiana Seed On Blood Glucose And Oxidative Stress In Serum And Liver In Diabetic Rats

Introduction: Diabetes can increase the oxidative stress in various tissues of the body, and the progress of this process is associated with the intensification of the complications of diabetes. The purpose of this study was to evaluate the protective effect of pinus gerardiana (PG)seed on oxidative stress induced by diabetes in liver and serum of STZ diabetic rats. Methods: This interventional study was performed on 36 male Wistar rats. The mice were randomly divided into 6 groups (healthy controls, healthy treated with 3 and 6% (PG), diabetic control, diabetic treated with 3 and 6% (PG) doses). After 6 weeks of intervention, weight, glucose and oxidative stress parameters in serum and liver including total antioxidant capacity, malondialdehyde, total thiol and superoxide dismutase activity were measured. Data analysis was done by statistical software version 16 and Tukeys one-way ANOVA tests. Results: Diabetic rats showed a significant increase in malondialdehyde and glucose levels and a significant decrease in weight and activity of superoxide dismutase enzymes, total thiol groups, and total antioxidant capacity of serum and liver (p < 0.001). Oral administration of PG seeds to diabetic rats caused a significant reduction in malondialdehyde and glucose levels and a significant increase in the weight and activity of superoxide dismutase enzymes, total thiol groups, and total antioxidant capacity of serum and liver (p < 0.001). Conclusion: The results of this study showed that 6-week treated with PG had a decrease in blood glucose and, by decreasing the oxidative complications of diabetes, is probably effective in preventing complications of diabetes. Key words: Diabetes mellitus, pinus gerardiana, oxidative stress, blood glucose, rat
Reza Malihi, Seyed Ahmad Hosseini, Maryam Vali