5TH International Congress on Technology - Engineering & Science - Kuala Lumpur - Malaysia (2018-02-01)

Antibacterial Activity Of Lactobacillus Casei On Growth And Enterotoxin Production Of Staphylococcus Aureus 29213

Food borne intoxications are kind of current problem in human society and most of them are caused by enterotoxins of Staphylococcus aureus.. Also probiotics (Lactic acid bacteria) have been known as health and immune system modulator. In this experiment we assessed the effects of Lactobacillus casei (108cfu/ml) on growth of Staphylococcus aureus (105cfu/ml) at 35°C in TSB. Then samples from the co cultured and control were pour plated in Baird parker agar and MRS agar to compute the bacterial count at 0, 8, 24, 48, 72 hours after incubation. We found that Lactobacillus casei inhibited the growth of Staphylococcus aureus 29213. Comparing to controls reduced number of S. aureus cells was about 2 logs in 35 °C. Enterotoxin production by S. aureus was also inhibited in 35 °C according to ELIZA rida screen test. Lactobacillus casei inhibited the production of entrotoxins A and/orC at 35 °C. In conclusion, the results of this study revealed the effectiveness of Lactobacillus casei in inhibition of Staphylococcus aureus growth and enterotoxin production proposed their potential application as antibacterial in food.
Abolfazl Kamkar, Ali Misaghi