5TH International Congress on Technology - Engineering & Science - Kuala Lumpur - Malaysia (2018-02-01)

Gold Nanoparticle-based Colorimetric Biosensors For The Detection Of Glycated Hemoglobin

We report novel biosensors for the highly sensitive and specific enzymatic analysis of glycated hemoglobin (HbA1c) that is measured primarily to identify the three-month average plasma glucose concentration. Hydrogen peroxide (H2O2) was generated in this enzymatic measuring of glycated hemoglobin from oxidative cleavage of fructosyl valine by fructosy amino acid oxidase (FAOD), the generated hydrogen peroxide was then used as a reducing agent of gold (III) ion for the synthesis of gold (0) as a nanoparticle. Gold nanoparticles obtained from this novel approach could be measured by optical methods using UV-VIS spectrophotometer. In optical method, we have carried out 96-well plate-based analysis of glycated hemoglobin and this system worked very well having the detection limit of 0.142% of glycated hemoglobin and coefficient of variation of below 5 %. Finally, this sensing method is available to measure the glycated hemoglobin level for diabetes patients and normal person (4 - 6% HbA1c).
Soo Suk Lee