5TH International Congress on Technology - Engineering & Science - Kuala Lumpur - Malaysia (2018-02-01)

In Silico Analysis Of Major Proteins From Salmonella Typhimurium Biofilm

Salmonella typhimurium, an opportunistic pathogenic bacterium, has become an emerging issue in food industries. Colonization of this microorganism on various surfaces forming biofilm often causes ineffective cleaning and sanitation. 25.4 kDa and 51.2 kDa proteins are the major proteins in S. typhimurium [1]. These proteins are continuously expressed across all developmental stages and are essential for microbial typing [1-2]. Many works focus on antimicrobial treatment to combat S. typhimurium [3-4], however, the roles of these major proteins in S. typhimurium biofilm remains not well investigated. Thus, the present study was performed to identify and characterize the 25.4 kDa and 51.2 kDa proteins from S. typhimurium biofilm. The biofilm was developed in 6-well microplate at 37OC for 24 hours whilst whole-cell protein extraction was performed using detergent lysis method. The biofilm proteins were analyzed using a combination of polyacrylamide gel electrophoresis, tandem mass spectrometry and bioinformatics. The results demonstrated that the 25.4 kDa and 51.2 kDa protein bands contained 29 and 46 proteins respectively. The majority of the identified proteins were predicted to associate with cytoplasm, general metabolism and significant (p<0.05) functional interaction networks. Further analysis using k-means clustering algorithm successfully produced four non-overlapping protein clusters. In conclusion, the biofilm proteins identified herein could be considered as important antimicrobial targets. References: [1] Yahya M F Z R, Alias Z, Karsani SA. Subtractive protein profiling of Salmonella typhimurium biofilm treated with dimethyl sulfoxide. The Protein Journal 2017; DOI: 10.1007/s10930-017-9719-9. [2] Aksakal A. Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE. Veterinarni Medicina 2010; 55(6): 259-263. [3] Tabak M, Scher K, Hartog E, Romling U, Matthews K R, Michael L, Chikindas M L, Yaron S. Effect of triclosan on Salmonella typhimurium at different growth stages and in biofilms. FEMS Microbiol. Lett. 2006; 267: 200–206. [4] Yahya M F Z R, Alias Z, Karsani SA. Antibiofilm activity and mode of action of DMSO alone and its combination with afatinib against Gram negative pathogens. Folia Microbiologica 2017; DOI: 10.1007/s12223-017-0532-9.
MOHD FAKHARUL ZAMAN RAJA YAHYA, ZAZALI ALIAS, SAIFUL ANUAR KARSANI