4TH International Congress on Technology - Engineering & SCIENCE - Kuala Lumpur - Malaysia (2017-08-05)

Study On Total Phenolic, Flavonoid Contents And Antioxidant Activity Of Water Extract Of Endemic Plant Satureja Hortensis Leaves

synthetic antioxidants such as butylated hydroxyanisole and butylated hydroxy toluene are restricted by legislative rules because they are suspected to have some toxic effects and as possible carcinogens. Keeping in this view, there has been a considerable interest by the industry and a growing trend in consumer preferences for natural antioxidants, in this study, total phenolics, flavonoids contents , antioxidant activity and the protective effects of water extract of Satureja hortensis L., in stabilizing soybean oil against oxidation at different concentrations (200 and 400 ppm) were tested.The aerial parts of S. hortensis L. were extracted with 100 ml water. Soybean oil was selected due to its unsaturated fatty acids variety. Antioxidant activities of the water extract of the Satureja hortensis was evaluated by peroxide values and 2-thiobarbituric acid tests in soybean oil for 49 days every 7 days. To assess the antioxidant efficacy of extract, the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and β- carotene/linoleic acid systems were used. Total phenolic and flavonoid contents of samples were investigated. Oxidation experiments were carried out in triplicates; results were averaged and statistically analyzed with one-way ANOVA by using SPSS 19. water extract could significantly lower the peroxide value and thiobarbituric acid value of oil (p≤0.05). The IC50 value for extract was 21.5 ± 0.7 µg/ml. In the β- carotene/linoleic acid system, aqueous extract exhibited 89.5 ± 1.20 % inhibitions against linoleic acid oxidation. The total phenolic and flavonoid contents of extract was (161.12 ± 0. 28 m g/ g) and (76.39 ± 0. 22 mg/ g), expressed in Gallic acid and Quercetin equivalents, respectively.the leaves of satureja hortensis could be a good source of antioxidant and water extract of satureja may have potential application as natural antioxidant in the edible oil and food industry.
Abolfazl Kamkar