International Congress on Engineering, Sciences and Innovative Technologies (ICESIT 2020) - Kuala Lumpur - Malaysia (2020-02-13)

Characterization of an efficient biosurfactant producing bacteria from sugar industry waste and their possible biotechnological uses. Page v
Abstract The current study was mainly focused on the screening of microorganisms from the wastewater of the sugar industry for the production of biosurfactants and their possible biotechnological uses. Amongst the 10 bacterial strains, S1 was an efficient biosurfactant producer. Morphological and microscopic examination showed that the strain belonged to Bacillus species. Results of various biosurfactant production tests revealed a considerable decrease in the surface tension from 69 to 32 while the emulsification index was also noted which was 75%. The biosurfactant was extracted by acid precipitation technique which was partially characterized as lipopeptide. Three types of culture media were used for the optimum growth activity which was measured by optical density at 600nm. Amongst the three culture media one was selected i.e. MSM1. Various conditions were optimized for the highest growth activity and i.e. pH, temperature and inoculum size result showed that the optimum growth activity was observed at 7 pH 370C temperature and 1% of the inoculum size at 48 hours of incubation time. Physical properties of the biosurfactant indicated that the compound was active in unfavorable condition. Soil washing activity was done by the compound produce by S1 bacterial strain which eliminate high number of hydrocarbon from the contaminated soil. Condition were optimized i.e. pH, temperature and shaking speed for the soil washing result showed that at pH 6, 60% of the hydrocarbon is removed from the contaminated soil. Temperature was also optimized at 60 oC where 80% of the contaminated was removed. The third parameter was shaking speed at 160rpm and the surfactant showed optimum activity.
Mr. TAQI RAZA, Mr. Baseer Khan