3RD INTERNATIONAL CONGRESS ON TECHNOLOGY - ENGINEERING & SCIENCE - Kuala Lumpur - Malaysia (2017-02-09)

Proteome Analysis Of Fusarium-resistant And Fusarium-susceptible Musa Acuminata Cultivars By Using Two-dimensional Gel Electrophoresis (2de)
Bananas are known as one of the major food crops that contain high nutritional sources. In many tropical and subtropical countries, banana including dessert and cooking types have been used as an important export commodity crops. However, the cultivation is threatened severely by lethal fungal disease due to its susceptibility towards soil-borne fungus Fusarium oxysporum f sp. cubense (Foc). Since not all cultivars of banana have the same level of resistance to Foc, therefore, understanding of the molecular interactions in banana defence responses toward pathogen infection is essential to discover the signalling processes that underline banana’s defence mechanisms. In this study, a proteomic approach was used to elucidate how a susceptible cultivar (Pisang Berangan) and a resistant cultivar (Pisang Lilin) respond to Foc during infestation. By using two-dimensional gel electrophoresis (2DE) method, the proteome profiles of the infected root of both resistant and susceptible banana cultivars were compared. In the resistant cultivar, a total of 40 protein spots showed a-fold change after inoculation compared with the control, while in the susceptible cultivar, 18 spots showed a-fold change after challenge with Foc. All of the proteins identified may be related to metabolism, energy, immunity and defence, and unknown function. To determine whether the observed proteome changes are associated with banana resistance, further analysis will be performed by MALDI-TOF MS for proteins identification and functional classification. This study suggested that banana resistance is linked to the abundance of pathogenesis-related (PR) proteins expressed.
Murni Miru, Nor Aisyah Mohd Nor Azmi, Alan Kang-Wai Mu, Mohd Puad Abdullah