3RD INTERNATIONAL CONGRESS ON TECHNOLOGY - ENGINEERING & SCIENCE - Kuala Lumpur - Malaysia (2017-02-09)

In Situ Green Synthesis Of Silver-nanoparticles/bacterial Cellulose Nanocomposites For Antimicrobial Wound Dressing
Bacterial nanocellulose (BNC) is known as an ideal wound dressing, but it exhibits no antibacterial efficiency by itself. Hence, in this study BNC was functionalized with green synthesized silver nanoparticles (AgNPs) to make an effective wound dressing with strong antimicrobial properties. AgNPs with different concentrations of Ag precursors were synthesized within BNC using Citrullus colocynthis aqueous extract. The BNC/AgNPs nanocomposites were characterized using FTIR, XRD, and FESEM analyses. XRD and FESEM results showed that spherical and crystalline AgNPs with a mean size of 32± 2.1 nm were generated and well dispersed in the BNC. FTIR spectra revealed the good interaction between AgNPs and BNC. Such hybrid nanostructure inhibited Ag nanoparticles from dropping off BNC network and hence minimized the toxicity of nanoparticles. Moreover, antimicrobial activity was evaluated on three types of gram positive and negative bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis by disk diffusion methods. Silver nanoparticles with different concentrations were able to inhibit 5.32 mm to 22.30mm size inhibition. Additionally, BNC/AgNPs a ready-to-use wound dressing for the treatment of infected wounds with excellent antibacterial activity.
Mona Moniri, Rosfarizan Mohamad, Raha Abdul Rahim, Wan Zuhainis Saad, Farideh Namvar