6TH International Congress on Technology - Engineering - Kuala Lumpur3 - Malaysia (2018-07-19)

Evaluation Of Ziziphora Clinopodioides Essential Oil And Whey Protein Isolate (wpi) Coating On Extend Shelf Life And Listeria Monocytogenes Control In Cube Beef

Ziziphora clinopodioides is the plant species that belonged to the Lamiaceae family and have significant antibacterial activity. Whey protein isolate (WPI; >90%) have been used as a coating solution for improving food quality and extending shelf life of some perishable foods. In this study ziziphora clinopodioides essential oil (EO) obtained by hydrodistillation method and chemical composition determined by GC/MS. Different concentrations of ziziphora clinopodioides essential oil (0, 0.6% and 1% v/v) were added to WPI solution. The initial inoculation of L. monocytogenes was 3.05 log cfu/g. cubic beef coated by WPI containing different concentrations of ziziphora clinopodioides EO and were packaged and stored at 4?c and total viable count (TVC), Psychrotrophic, pseudomonas spp. and L. monocytogenes were investigated in 0, 3, 6, 9 and 12 days of storage. The GC/MS results showed 22 organic compound representing 97.29% of total identified EO and thymol (30.67%), borneol (12.78%) and p-cymene (7.92%) were major compound of ziziphora clinopodioides EO. Result of this study showed that TVC of control treatment in 6th of study and the treatment coated with 0.6% and 1% of ziziphora clinopodioides EO in 9th and 12th of study, respectively, were exceed than 7 log cfu/g that showed a significantly antimicrobial effects and extend shelf life on the antimicrobial coated treatments. Also results showed Psychrotrophic and pseudomonas spp. count were significantly decreased in cube beef coated with WPI containing 0.6% and 1% ziziphora clinopodioides EO in comparison to control samples during study period. L. monocytogenes on 12th of investigation showed a 2.15 and 2.59 log cfu/gr reductions due to coated the cube beef with WPI contain 0.6% and 1% ziziphora clinopodioides EO in comparison to control treatment and demonstrated control effect of ziziphora clinopodioides EO on L. monocytogenes in cube beef stored at 4?c. In conclusion, results of this study revealed WPI coating containing ziziphora clinopodioides EO can increase shelf life and safety of cube beef.
Elmira Vanaki, Nima Babolani mogadam