5TH International Congress on Technology - Engineering & Science - Kuala Lumpur - Malaysia (2018-02-01)

In Vivo And In Silico Toxicity Assessment Of Hexane Rhizome Extract From Black Turmeric (curcuma Caesia Roxb).

Purpose: Curcuma caesia Roxb (CC) from zingeberacea family is commonly used food preservative spice. Many medical properties are associated to this spice. Small molecules present in the plant are responsible for its various medicinal properties. Some of these natural products may be toxic. Understanding the toxicity of Hexane Rhizome Extract (HRE) will provide useful information that may act as a reference for nutraceutical developments. Methods: The methodology focuses on developing an integrated in vivo and in silico approach for natural product drug discovery that combines bioactivity screening in zebrafish embryos and rapid computational toxicity predictions. Result(s): Extract of CC (1µg – 5 µg) was administered to screen the toxicity in zebrafish embryos. Only higher concentrations of 5 µg showed considerable mortality. In the in silico prediction, 80% of the 20 small molecules identified in HRE were found to be mutagenic and carcinogenic. Conclusion(s): These results suggest that the combination of zebrafish bioassays with predictive toxicity methods is an effective strategy for the researchers to design and conduct preclinical studies in a timely and cost-effective way. Keywords: Curcuma caesia Roxb, Zebra fish, In silico Toxicity Reference: Mukunthan KS, Satyan RS, Patel TN. Pharmacological evaluation of phytochemicals from South Indian Black Turmeric (Curcuma caesia Roxb.) to target cancer apoptosis. J Ethnopharmacol. 2017, 209:82-90.
Mukunthan KS, Patel TN