5TH International Congress on Technology - Engineering & Science - Kuala Lumpur - Malaysia (2018-02-01)

Impact Of Silver And Copper Nanoparticles On Fusarium Solani F. Sp. Phaseoli Causal Bean Dry Root Rot Disease

Dry root rot caused by Fusarium solani f. sp. phaseoli is an important soil-borne bean disease in the world. The pathogen isolated, purified and identified from a bean field in south-west Iran. Its pathogenicity was investigated by inoculation of spores suspension with concentration of 106/ml to the root of young seedlings of white bean in the greenhouse. Once the symptoms of the disease were appeared, from the rotted root tissues, the fungus was isolated and identified again. Impact of silver and copper nanoparticles on colony growth of the pathogen, evaluated with mix 1000, 300, 100, 50, 30, 10 ppm concentration of these nanoparticles with potato-dextrose-agar medium in vitro. After seven days, the growth of the pathogen colony was measured in each treatment and compared with control. Then effective concentration for 50% inhibition of colony growth of the pathogen (EC50) of each nanoparticle determined. Silver and copper nanoparticles had EC50=25,126 ppm for colony growth of the pathogen. Therefor silver nanoparticle has higher impact on growth of this pathogen and can use for control of this important soil-borne bean disease.
Mehdi Sadravi