5TH International Congress on Technology - Engineering & Science - Kuala Lumpur - Malaysia (2018-02-01)

Antimicrobial Activity Of Poly Lactic Acid Films Containing Ziziphora Clinopodioides Essential Oil Against Bacillus Subtilis And Salmonella Typhimurium

Innovative active food packaging containing natural antimicrobial agents such as essential oils is an important technique to reduce the use of chemicals preservatives in food. In this research, antimicrobial activity of poly lactic acid films containing different concentrations of Ziziphora clinopodioides essential oil (0, 1 and 2 %) was investigated against B. subtilis and S. typhimurium by applying disk diffusion method. PLA films containing different concentration of Ziziphora clinopodioides essential oil prepared by casting method on glass petri dishes. Poly lactic acid films containing Ziziphora clinopodioides essential oil were cut to circular discs (6 mm diameter). Then disks were placed on Muller-Hinton agar plates that previously inoculated by 1×108CFU/ml of B. subtilis and S. typhimurium. Inhibition zones were measured after 24 h incubation of plates at 35o C, by using Digital Caliper. The statistical analysis was done by one way analysis of variance (ANOVA) followed by Tukeys test. Result of this study showed that pure PLA films did not show any inhibitory effect against tested bacteria whereas incorporation of essential oil to the films result in antimicrobial activity. Incorporation of 2 % concentrations Ziziphora clinopodioides essential oil to the poly lactic acid film solution lead to inhibition zones of 13.52 ± 0.19 and 10.24 ± 0.36 for B. subtilis were and S. typhimurium, respectively. In conclusion poly lactic acid films containing Ziziphora clinopodioides essential oil have a good potential to apply as an antimicrobial food packaging material.
Afshin Akhondzadeh Basti, Ali Khanjari, Ali Misaghi, Peyman Mahasti Shotorbani, Yasser Shahbazi, Nassim Shavisi