4TH International Congress on Technology - Engineering & SCIENCE - Kuala Lumpur - Malaysia (2017-08-05)

Testing For Resistance Of Db1 Transgenic Rice Line And Others To Two Brown Planthoppers (nilaparvata Lugens Stal.) Biotypes
Brown planthopper (BPH, Nilaparvata lugens Stal.) is well-known as a major pest of rice crop. Developing rice resistant to BPH is economic and environmental friendly approach. Transgenic rice line with DB1 (Dioscorea batatas tuber lectin 1) transgene has been engineered to overcome the problem. The objective of the experiment was to obtain the level of resistance of a DB1 rice line (cv. Taichung 65 inserted with DB1) and some rice genotypes against two colonies of BPH. Testing for resistance was performed in BPH’s preference, honey dew test, crop damage and population build-up. Two biotypes of BPH i.e., biotype 2 (colony Sukamandi) and biotype 3 (North Sumatera) were applied. The result showed that transgenic rice with Db1 was resistant to biotype 2, while to be susceptible when invested to biotype 3. However the DB1 could increase one resistance level of rice cv. Taichung 65 against biotype 2 of BPH, from susceptible to moderately resistance. PTB-33 and Rathu Heenati were highly resistance to these two biotypes. Number of brown planthoppers was significantly different among cultivars. This is the first report notifying the resistant level of DB1 transgenic rice line for both biotypes of BPH in Indonesia.
Nono Carsono, Gigih Ibnu Prayoga, Danar Dono, Santika Sari, Diani Damayanti,